ISSN 1524-0215
Vol. 25, Issue Suppl, 2014May 01, 2014 EDT
Precise Quantification of Next Generation Sequencing Ion Torrent™ and Illumina Libraries using the QuantStudio™ 3D Digital PCR Platform
Precise Quantification of Next Generation Sequencing Ion Torrent™ and Illumina Libraries using the QuantStudio™ 3D Digital PCR Platform
Schweitzer P., Harris A., Mandelman D., Jackson S., Cifuentes F., Degoricija L.,
Articles in Vol. 25, Issue Suppl, 2014
Vol. 25, Issue Suppl, 2014
- Strand-Specific Transcriptome Sequencing for Challenging SamplesFish RachelGandlur SuvarnaFarmer AndrewBetts Craig
- Development of a Luminex Immunobead-Based Method for Autoantibody DiscoveryFhied CristinaPiper ClintPithadia RaviLiptay Michael J.Borgia Jeffrey A.Pergande Melissa
- Impact of the Development of a Light Microscopy Shared Resource for the University of Rochester Medical Center: A Quantitative AssessmentJepson M.Jordan P.Kasischke K.Brown E.Reed A.Lentine M.Bushnell T.Puzas E.Callahan L.M.
- Case Study: Regulatory Considerations in the Analysis of Human Patient Samples in an Academic Core LabKrasinska K.M.McLaughlin T.M.Ingrande J.Chien A.S.
- Rapid DNA-Seq to Achieve High Coverage Libraries from 1ng – 1 μg in 2 HoursDickman J.Toloue M.M.Risinger J.
- Identification of the Free Cysteinyl Residue and Characterization of the N-glycan Structure in the C-terminal Domain of PCSK9Robinson John H.Spahr ChrisShi Stone D.-H.Wang WeiLu Hsieng S.Hui John O.
- A Novel Approach for Processing LC – Ion Mobility – MS Metabolomics DataAstarita GiuseppePalmer MartinBennett MarkLangridge James I.Shockcor John P.Borthwick AndyIsaac Giorgis
- Separation of N-Glycans with Different Sialic Acid Linkages by Using Novel Superficially Porous Particle HILIC ColumnHuang Y.Boyes B.Orlando R.Tao S.
- Operation of the Advanced Instruments at the Flow Cytometry Resource Center: Extensive User Training vs. Staff-Assistant ServiceMazel S.
- Overview of Production of Protein Using Cell-free SystemsGao Fei Philip
- Biomedical Engineer's Role in Improving the Management of Devices Used for Genomic Medicine ResearchGutierrez O.Hernandez B.
- A Workflow of MS/MS Data Analysis to Maximize the Peptide IdentificationXing LeiYang LiangShan BaozhenZhang Zefeng
- Precise and Accurate Determination of microRNA Precursors by Digital PCRMead E.Wang Y.Patricia H.Jackson S.Pietrzykowski A.Patel S.
- Transcriptome Analysis from Low Cell Numbers: Two RNA-Amplification ApproachesVallandingham J.Peak A.Morrison J.Bailey C.Staehling K.Perera A.Zueckert-Gaudenz K.Kulesa P.Li H.Fleharty B.
- Characterizing Low Stoichiometry Phosphorylation Events without the Requirement for Extensive FractionationThompson J.W.Farnsworth C.L.Silva J.C.Moseley M.A.Soderblom E.J.
- Metabolomics Investigation of Spiked Compound Differences in Human PlasmaAsara JohnNeubert ThomasTurck ChrisCheema Amrita K
- Strategies for Profiling Single Mouse Intestinal Epithelial Cells by Targeted Gene ExpressionMcDowell W.Box A.Staehling K.Wang F.Li L.Zueckert-Gaudenz K.
- Secondary Structures of Plasmodium falciparum Associated with Recombination in Variable Surface Antigen Gene FamiliesQaadri K.Olsen C.
- Evaluating Effects of Cell Sorting on Cellular IntegrityLopez P.Bergeron A.Box A.Brundage K.Chittur S.Cochran M.Meyer E. M.Tighe S.DeLay M.
- Computing Multi-Level Clustered Alignments of Gene-Expression Time-SeriesCraven MarkMuganda-Rippchen Deborah
- Characterizing Sequencing Samples with k-mer Spectra for Good and EvilOwens S.Keegan K.Meyer F.Trimble W. L.
- Reference Proteome Extracts for Mass Spec Instrument Performance Validation and Method DevelopmentRosenblatt MikeUrh MarjetaSaveliev Sergei
- Alternate Cluster Generation for Picomolar NGS LibrariesHuerta G.Heckmann T.Deiderick H.Barnette M.Hunicke-Smith S.P.Podnar J.W.
- Optimization of Capital Research Equipment Usage through RED-iness of InformationShimanuki M.Briones A. Villar
- Opening the Doors to Pre-Commercial TechnologyGeorge Reed A.
- Automated Big Data Analysis in Bottom-up and Targeted Proteomicsvan der Plas-Duivesteijn SuzanneDomański DominikSmith DerekBorchers ChristophPalmblad MagnusMohamme Yassene
- An Improved cDNA Library Generation Protocol for Transcriptome Analysis from a Single CellFish RachelZhang SallyBostick MagnoliaChang CynthiaGandlur SuvarnaFarmer Andrew
- Detecting and Quantifying Low Level Gene Variants in Sanger Sequencing Traces Using the ab1 Peak Reporter ToolRoy S.Schreiber E.
- Effects of RNA Degradation on Performance of the Illumina Whole-Genome DASL HT AssayLi X.Islam MDByers S.W.Riggins R.Goerlitz D.
- Genomics Research Group SessionBaldwin D.Chittur S. V.Raghavachari N.Jafari N.Aquino C.Perera A.Reyero N.G.
- Lessons Learned: A Guide for Implementing and Activating a Centralized Billing SystemMays L.White A. N.
- Optimization of Library Construction Protocol to Sequence Large Fragment Libraries on PacBioPanayotova N.G.Zhou X.H.Yuan GMoraga D.A.Shanker S.
- Nucleic Acid Research Group 2013–2014 Study: Evaluating Library Synthesis Protocols for Sub-Nanogram ChIPSeq samplesCarmical J. RussLytle Christian HNadella VijayanandBeckloff NicholasPerera AnojaTighe ScottChittur Sridar VHerbert ZachAuer Herbert
- Improvement of OMSSA for High Accuracy MS/MS DataAzencott R.Hawke D.H.Kong A.
- A Next Generation Sequencing PCR Primer Design Tool for Sanger Sequencing Confirmation by CEBerosik S.Wenz M.Brzoska P.Schreiber E.Hu F.You X.Lam H.Sovan S.Lim S.-H.Gordeeva K.Ho A.Karger A.
- Nucleic Acid Research Group 2013–2014 Study: Evaluating Library Synthesis Protocols for Sub-Nanogram ChIPSeq samplesCarmical J. RussLytle Christian HNadella VijayanandBeckloff NicholasPerera AnojaTighe ScottChittur Sridar VHerbert ZachAuer Herbert
- A Biosafety Concerns and Solutions for Safe Cell SortingLopez P.Gregory M.
- Deep Sequencing of a Recent Bacterium Found in the Human Oral CavityGhadiri J.Garcia S.Caldwell A.Vinnichenco I.Ouverney C.Ghadiri F.
- SOMAscanTM v3.0: A Sensitive and Reproducible, Multiplex Proteomic Platform that Measures over 1100 Analytes in Complex MatricesKraemer S.Sanders G.Katilius E.King E.Bauer T.Zichi D.Saccomano N.Lollo B.
- Locked Nucleic Acid and TaqMan Probes to Detect KRAS Mutations by Droplet Digital PCR in Colorectal Carcinoma Formalin Fixed Paraffin Embedded Tissue SamplesChapman B.Brown A.Cooper S.Yang W.Karlin-Neumann G.Binder S.Sullivan D.C.
- Antibody Research GroupBagarozzi DennisBrundage KathrynCarnahan RobertCrimmins Dan L.Weis-Garcia FrancesBlum David L.
- Reducing Sample Amounts for Isobaric Tagging Quantitative Proteomics ExperimentsLoprest M.Colangelo C. M.Abbott T. B.
- Combining a Chip-Mate nESI Ion Source with Make-Up Flow Strategy for Increased System Robustness and Sensitivity in nLC-nESI-MSWang C.Jones J.Prosser S.J.Eikel D.
- A Microscope Evaluation Database for the Large Microscopy CoreWiegraebe WinfriedHoffman Steven J
- Comparison of Commonly Used Methods for Protein Relative Quantification in Complex SamplesChoksawangkarn WaeowaleeDhabaria AvantikaWang Yan
- Impact of the Development of a Light Microscopy Shared Resource for the University of Rochester Medical Center: A Quantitative AssessmentJepson M.Jordan P.Kasischke K.Brown E.Reed A.Lentine M.Bushnell T.Puzas E.Callahan L.M.
- Case Study: Regulatory Considerations in the Analysis of Human Patient Samples in an Academic Core LabKrasinska K.M.McLaughlin T.M.Ingrande J.Chien A.S.
- A Practical Evaluation of Next Generation Sequencing & Molecular Cloning SoftwareQaadri K.Moir R.Kearse M.Buxton S.Wilson A.Cheung M.Milicevic B.Hengjie W.Kuhn J.Stones-Havas S.Olsen C.
- Next Generation Sequencing Analysis of Circulating RNAs in Human PlasmaSu XiaopingTang HongliRamagli LouisSun SipingThompson ErikaKhanna Kanhav
- A Molecular Indexing for Improved RNA-SeqToloue M.M.Risinger J.
- Automated Assessment of Next Generation Sequencing Library Preparation Workflow for Quality and Quantity Using the Agilent 2200 TapeStation SystemBorg SolangeSalowsky RuedigerPadmanaban ArunkumarSaligrama DeepakWalker Donna McDadeInche AdamElliott JimLiu Melissa Huang
- An Integrative Approach for Interpretation of Clinical NGS Genomic Variant DataStabley D. L.Chen C.Huang H.Robbins K.Polson S.Sol-Church K.Wu C.Crowgey E. L.
- Characterizing Low Stoichiometry Phosphorylation Events without the Requirement for Extensive FractionationThompson J.W.Farnsworth C.L.Silva J.C.Moseley M.A.Soderblom E.J.
- Metabolomics Investigation of Spiked Compound Differences in Human PlasmaAsara JohnNeubert ThomasTurck ChrisCheema Amrita K
- Strategies for Profiling Single Mouse Intestinal Epithelial Cells by Targeted Gene ExpressionMcDowell W.Box A.Staehling K.Wang F.Li L.Zueckert-Gaudenz K.
- Precise Quantification of Next Generation Sequencing Ion Torrent™ and Illumina Libraries using the QuantStudio™ 3D Digital PCR PlatformSchweitzer P.Harris A.Mandelman D.Jackson S.Cifuentes F.Degoricija L.
- Antibody Fluorophore Conjugation: A Simple Cell-Free Method for Quality ControlRuisaard K.Ramirez K.Dwyer D.Bonilla D.Jewell R.Clise-Dwyer K.MS K. Acklin
- Machine Learning Algorithms Implemented in Image AnalysisChen J.Renner L.Neuringer M.Cornea A.
- The erccdashboard: an R Package for Analysis of External Spike-in RNA Controls in Gene Expression ExperimentsLund S.Pine P.S.Salit M.Munro S.A.
- Evaluating Effects of Cell Sorting on Cellular IntegrityLopez P.Bergeron A.Box A.Brundage K.Chittur S.Cochran M.Meyer E. M.Tighe S.DeLay M.
- Computing Multi-Level Clustered Alignments of Gene-Expression Time-SeriesCraven MarkMuganda-Rippchen Deborah
- Characterizing Sequencing Samples with k-mer Spectra for Good and EvilOwens S.Keegan K.Meyer F.Trimble W. L.
- Reference Proteome Extracts for Mass Spec Instrument Performance Validation and Method DevelopmentRosenblatt MikeUrh MarjetaSaveliev Sergei
- Alternate Cluster Generation for Picomolar NGS LibrariesHuerta G.Heckmann T.Deiderick H.Barnette M.Hunicke-Smith S.P.Podnar J.W.
- Optimization of Capital Research Equipment Usage through RED-iness of InformationShimanuki M.Briones A. Villar
- Evaluation of Billing and Tracking Programs Leads to a Hybrid ApproachRupert A.White A.N.Wagner M.Thornton S.DeLay M.
- Longer Reads Do Not Significantly Improve RNA-seq ResultsChhangawala SagarRudy GabeWu Po-YenTighe ScottWang May D.Baldwin Don A.Grills GeorgeMason Christopher E.the ABRF-NGS ConsortiumRosenfeld Jeffrey
- The ABRF Next Generation Sequencing Study: Multi-platform and Cross-methodological Reproducibility of Transcriptome Profiling by RNA-seqLi ShengTighe ScottNicolet CharlesBaldwin DonGrills Georgethe ABRF-NGS ConsortiumMason Christopher E.
- Effect of Probiotic and Pathogenic Bacteria on Drosophila Intestinal PathologyHegan P.Mooseker M.Handelsman J.Miles J.
- Enabling High-Throughput Discovery of the RNA Transcription Landscape Using a Directional RNA Workflow and a Combinatorial Multiplexing ApproachMunafo Daniela B.Liu PingfangSumner Christine J.Langhorst Bradley W.Dimalanta Eileen T.Davis Theodore B.Stewart Fiona J.
- A Biosafety Concerns and Solutions for Safe Cell SortingLopez P.Gregory M.
- Deep Sequencing of a Recent Bacterium Found in the Human Oral CavityGhadiri J.Garcia S.Caldwell A.Vinnichenco I.Ouverney C.Ghadiri F.
- SOMAscanTM v3.0: A Sensitive and Reproducible, Multiplex Proteomic Platform that Measures over 1100 Analytes in Complex MatricesKraemer S.Sanders G.Katilius E.King E.Bauer T.Zichi D.Saccomano N.Lollo B.
- Locked Nucleic Acid and TaqMan Probes to Detect KRAS Mutations by Droplet Digital PCR in Colorectal Carcinoma Formalin Fixed Paraffin Embedded Tissue SamplesChapman B.Brown A.Cooper S.Yang W.Karlin-Neumann G.Binder S.Sullivan D.C.
- Antibody Research GroupBagarozzi DennisBrundage KathrynCarnahan RobertCrimmins Dan L.Weis-Garcia FrancesBlum David L.
- A Simple Multiplex PCR Approach for Target Enrichment in Next-Gen SequencingZhu QiHebel ChrisZhou Xiaochun
- Keeping Genomic Data Safe on the CloudRilak Z.Wernicke S.Bogicevic I.
- Comparison of Illumina and Ion Torrent RNA-Sequencing and Microarray-based approaches for Profiling the TranscriptomeKusko RebeccaLeClerc AshleySwahn SamanthaLenburg MarcSpira AvrumAlekseyev Yuriy
- Changing of the Guard: Migrating 16S and ITS Diversity Profiling from 454 Onto Alternate NGS PlatformsMcNally R.Johnson M.Kasinadhuni N.Tinning M.McGrath K.
- Accurate Genome Analysis of Single CellsFisch E.Fricke E.Baedker S.Deutsch U.Ahmed R.Loeffert D.Korfhage C.
- Reducing Sample Amounts for Isobaric Tagging Quantitative Proteomics ExperimentsLoprest M.Colangelo C. M.Abbott T. B.
- Combining a Chip-Mate nESI Ion Source with Make-Up Flow Strategy for Increased System Robustness and Sensitivity in nLC-nESI-MSWang C.Jones J.Prosser S.J.Eikel D.
- A Microscope Evaluation Database for the Large Microscopy CoreWiegraebe WinfriedHoffman Steven J
- Comparison of Commonly Used Methods for Protein Relative Quantification in Complex SamplesChoksawangkarn WaeowaleeDhabaria AvantikaWang Yan
- Investigation of Different Hierarchal Clustering Approaches for Protein Identification Directly from Tissue Section in a MALDI Imaging ExperimentCole LauraClench MalcolmClaude EmmanuelleTowers Mark
- Expression Profiling Smackdown: Human Transcriptome Array HTA 2.0 vs. RNA-SeqPalermo MeghannDriscoll HeatherTighe ScottDragon JulieBond JeffShukla ArtiVangala MaheshVincent JamesHunter Tim
- Sequencing Single Human and Bacterial Cells at Low Coverage for Aneuploidy, CNV, and Genotyping ApplicationsTesmer T.Yerramilli S.Carey M.Langmore JCarroll M.Kamberov E.
- Optimization of 384-well Luminex Immunoassays on the FlexMAP 3D System for Routine Analyte Quantitation from Commercial KitsPergande MelissaPithadia RaviBorgia Jeffrey A.Fhied Cristina
- Comparison of Fractionation Strategies Post-Enrichment by Titania Used in the Study of the Phosphoproteome of a Green AlgaMarsh E.L.Alvarez S.Evans B.S.Naldrett M.J.
- A Practical Example of Bringing Computation to DataDowns B.N.Opheim D.M.Hale W.Xi L.Donehower L.A.Kalra D.
- A Novel Analytical Pipeline for de novo Haplotype Phasing and Amplicon Analysis using SMRT® Sequencing TechnologyBowman B.Ranade S.Harting J.Lleras R.
- Requirements for Bacteriophage Growth: Using High Throughput Sequencing to Determine Gene EssentialityDedrick RebekahRubin MichaelHatfull GrahamPérez Joseph
- The HVAC Challenges of Upgrading an Old Lab for High-end Light MicroscopesRichard R.Martone P.Callahan L.M.
- Next-Generation Genomics Facility at C-CAMP: Accelerating Genomic Research in IndiaS ChandanaRussiachand HeikhamH PradeepS ShilpaM AshwiniS SahanaB JayanthAtla GouthamJain SmitaArunkumar NandiniGowda Malali
- Rapid DNA-Seq to Achieve High Coverage Libraries from 1ng – 1 μg in 2 HoursDickman J.Toloue M.M.Risinger J.
- Identification of the Free Cysteinyl Residue and Characterization of the N-glycan Structure in the C-terminal Domain of PCSK9Robinson John H.Spahr ChrisShi Stone D.-H.Wang WeiLu Hsieng S.Hui John O.
- A Novel Approach for Processing LC – Ion Mobility – MS Metabolomics DataAstarita GiuseppePalmer MartinBennett MarkLangridge James I.Shockcor John P.Borthwick AndyIsaac Giorgis
1.
P. S, A. H, D. M, S. J, F. C, L. D. Precise Quantification of Next Generation Sequencing Ion TorrentTM and Illumina Libraries using the QuantStudioTM 3D Digital PCR Platform. JBT. 2014;25(Suppl):S15-S15.